BOND 4-Plex Immunofluorescent Detection

The BOND 4‑Plex Immunofluorescent Detection Kit RUO enables high‑quality multiplex IF staining of FFPE tissues on the BOND RX and BOND RX^m research stainers. This pre‑assembled kit streamlines complex workflows by reducing manual preparation and minimizing human error. Powered by tyramide signal amplification, the kit provides high sensitivity and clear fluorescence signals, even for low‑abundance biomarkers, across four fluorophores (490, 550, 595, and 650 nm). A gentle elution buffer preserves tissue morphology and antigen integrity, supporting reliable data generation. With optimized automated protocols, researchers achieve consistent, reproducible multiplex staining while accelerating time to insight.

Kit Components:
The kit includes peroxide block, post primary, polymer, IF490, IF550, IF595, and IF650 fluorophore concentrates, along with an elution buffer and DAPI solution, all provided in a pre‑assembled format designed to minimize preparation time and ensure consistent performance.