Amplification of the human protooncogene, v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog (MYCN) is frequently seen either in extrachromosomal double minutes or in homogeneously staining regions of aggressively growing neuroblastomas. MYCN amplification has been defined by the INRG as > 4-fold MYCN signals compared to 2q reference probe signals. The MYCN (2p24) FISH probe is optimized to detect copy numbers of the MYCN gene region at 2p24. The AFF3 gene region probe at 2q11 is included to facilitate chromosome identification.
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