The CD34 antigen is a single chain transmembrane glycoprotein with a molecular weight of 110 kD. The CD34 protein is selectively expressed on human lymphoid and myeloid hemopoietic progenitor cells. The CD34 antigen is also expressed on vascular enothelium.
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Enzyme digestion of paraffin sections is recommended with clone QBEnd/10 in perference to heat induced epitope retrieval as it produces stronger staining and reduces background elastin staining
CD34 (Endothelial Cell Marker) is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains.