RUO - BCL2 Break

Follicular lymphoma is a mature B-cell lymphoma characterized by the presence of the t(14;18) translocation that juxtaposes the BCL2 locus on chromosome 18q21 to the immunoglobulin H (IGH) locus on chromosome 14q32, resulting in the overexpression of the anti-apoptotic protein BCL2. Next to IGH, other translocation partners to BCL2 are also known (e.g. IGK at 2p11.2 and IGL at 22q11). A break or split assay is therefore best suited to detect rearrangements of the BCL2 gene region at 18q21. The BCL2 (18q21) Break FISH probe is optimized to detect translocations involving the BCL2 gene region at 18q21 in a dual-color, split assay on metaphase/interphase spreads, blood smears and bone marrow cells.

References:
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Einerson R et al, 2005, Am J Clin Pathol, 124; 421-429.