Fluorescent in situ hybridization (FISH) identifies or labels target genomic sequences so that their location can be studied. DNA sequences from appropriate chromosome specific probes are first labeled with reporter molecules. The labeled DNA probe is then hybridized to the metaphase chromosomes or interphase nuclei on a slide. After washing, the specimen is screened for the reporter molecules by fluorescence microscopy.
For use on metaphase and interphase cells from peripheral blood cultures or direct preparations prepared by standard cytogenetic methods see: The ACT cytogenetics laboratory manual. 2nd ed. New York: Raven Press; 1991.
KBI-60004: This pretreatment kit is specifically developed to obtain optimal results on heavily cross-linked or difficult paraffin embedded tissues (e.g. longer fixation or prolonged storage).
KBI-60005: This pretreatment kit is specifically developed to obtain optimal results on FRESHLY prepared cytological samples.
KBI-60006: This pretreatment kit is specifically developed to obtain optimal results in case older samples, slides with cytoplasmic background, or difficult samples are used or expected. Note: freshly prepared cytological samples It is advised to use KBI-60005 FISH reagent kit in case freshly prepared cytological samples are used.
KBI-60007: This pretreatment kit is specifically developed to obtain optimal results on conventional paraffin-embedded tissues.
For paraffin embedded tissues it is recommended to use pretreatment kits KBI-60004 or KBI-60007.
FISH Digestion Kit
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