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Human tonsil: in situ hybridization using RNA Positive Control, Anti-Fluorescein Antibody and Bond Polymer Refine Detection.
Human tonsil: in situ hybridization using RNA Positive Control, Anti-Fluorescein Antibody and Bond Polymer Refine Detection.
BOND RNA Positive Control Probe
RNA Positive Control Probe is intended for use as a positive control in formalin-fixed, paraffin-embedded tissue by in situ hybridization (ISH) using the automated BOND system. RNA is very susceptible to degradation by RNases, therefore, the RNA Positive Control Probe is ideally used as a screening tool to detect the preservation of mRNA in cells.
RNA Positive Control Probe is an oligonucleotide probe designed to hybridize with the Poly(A) tail of messenger RNA (mRNA). The RNA Positive Control Probe is generated with a fluorescein label using the same procedures as applied to other oligonucleotide probes used in the detection of RNA on BOND.
Staining with the RNA Positive Control Probe should result in dark brown nuclear staining with some cytoplasmic staining, depending on the translational activity of the cell.
