GLI1 (12q13) / SE 12 hybridized to patient material showing GLI (12q13) amplification (3R2G). Image kindly provided by Dr. Wenzel, Basel.
IVD GLI1/SE 12
Trisomy 12 is the most common numerical chromosomal aberration in patients with B-cell chronic lymphocytic leukemia (B-CLL). Partial trisomy 12 of the long arm of chromosome 12 consistently includes a smaller region at 12q13-15 and has been observed in CLL and several other tumors. A number of loci located close to either MDM2 or CDK4/SAS, including the genes GADD153, GLI1 (previously known as GLI), RAP1B, A2MR, and IFNG, were found to be coamplified. The GLI1 (12q13) specific FISH probe is optimized to detect copy numbers of the GLI1 gene region at region 12q13. The chromosome 12 Satellite Enumeration FISH probe (SE 12) D12Z3 is included to facilitate chromosome identification.
References: Merup et al, 1997, Eur J Haematol, 58; 174-180. Dierlamm et al., 1997, Genes Chrom Cancer, 20; 155-166.