Hybridization of BCL6 break probe to a tissue section showing aberrant pattern (1RG1R1G).
IVD BCL6 Break (tissue)
Chromosomal translocations involving band 3q27 with various different partner chromosomes represent a recurrent cytogenetic abnormality in B-cell non-Hodgkin's lymphoma. Kreatech has developed this probe for the specific use on cell material (KBI-10607), or on tissue (KBI-10730). Two different breakpoint regions have been identified; the major breakpoint region (MBR) is located within the 5' noncoding region of the BCL6 proto-oncogene, while the atypical breakpoint region (ABR) is located approximately 200 kb distal to the BCL6 gene. The BCL6 (3q27) Break probe is designed to flank both possible breakpoints, thereby providing clear split signals. The BCL6 (3q27) Break probe is optimized to detect translocations involving the BCL6 gene region at 3q27 in a dual-color, split assay on paraffin-embedded tissue sections.
References: Butler et al, 2002, Cancer Res, 62; 4089-4094. Sanchez-Izquierdo, 2001, Leukemia, 15; 1475-1484.