BCL2 / IGH t(14;18) probe hybridized to a normal interphase/metaphase (2R2G).
The t(14;18) chromosomal translocation is a common cytogenetic abnormality in human lymphoma and is observed in about 85% of follicular lymphoma (FL) and up to one-third of diffuse lymphomas (DL). Two breakpoint region clusters (brc) have been identified: a major breakpoint region (mbr) within the 3' untranslated region of the BCL2 proto-oncogene (approximately 60% of the cases) and a minor cluster region (mcr) 30 kb 3' of BCL2 ( approximately 25%). The BCL2/IGH t(14;18)(q21;q32) specific FISH probe is optimized to detect the reciprocal translocation t(18;14), involving either of the two brc in the BCL2 gene in a dual-color, dual-fusion assay. Kreatech has optimized this FISH probe for the specific use on cell material (KBI-10606), or on tissue (KBI-10755).
References: Poetsch et al, 1996, J Clin Oncol, 14; 963-969. Vaandrager et al, 2000, Genes Chrom Cancer, 27; 85-94.