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IHC/ISH Super Blocking is specially formulated to stabilize antibodies and eliminate background encountered in immunohistochemistry (IHC) and in situ hybridization (ISH) procedures. The diluent contains complex protein and non-protein mixtures that eliminate background by blocking non-specific protein-protein interactions.
It is particularly recommended for staining of mouse tissues with mouse primary antibodies. It can also be used as a diluent for primary antibodies. If primary antibodies are diluted in this solution, a blocking step before primary antibodies can be omitted.