Human tonsil: in situ hybridization using RNA Negative Control, Anti-Fluorescein Antibody and BOND Polymer Refine Detection.
Human tonsil: in situ hybridization using RNA Negative Control, Anti-Fluorescein Antibody and BOND Polymer Refine Detection.
BOND RNA Negative Control Probe
RNA Negative Control Probe is intended for use in the identification of background staining resulting from non-specific interactions in formalin-fixed, paraffin-embedded tissue by in situ hybridization (ISH) using the automated BOND system.
RNA Negative Control Probe is a single oligonucleotide, designed from zebra fish DNA and analysed using Basic Local Alignment Search Tool (BLAST) analysis to confirm that the sequence bears no homology with any human sequences. The RNA Negative Control Probe is generated with a fluorescein label using the same procedures as applied to other oligonucleotide probes used in the detection of RNA on BOND. Therefore, RNA Negative Control Probe is ideal as a negative control probe for RNA ISH on BOND.
