The PreimpScreen Blas probes are designed for the use on human polar body or blastomere cells. It consists of a 5-color, five probe mixture of DNA probe sequences homologous to specific regions on chromosomes 13, 18, 21, X, and Y. Each of the probes is directly labeled with one of Kreatech’s ULS- fluorophores:
Critical region 1 (dark red): The 13q14 region probe is direct-labeled with PlatinumBright ™590. Critical region 2 (green): The 21q22 region probe is direct-labeled with PlatinumBright ™495. Critical region 3 (light red): The Xp11 region probe is direct-labeled with PlatinumBright ™547 Critical region 4 (blue): The Yq11 probe is direct-labeled with PlatinumBright ™415. Critical region 5 (dark blue): The Satellite Enumeration 18 probe is direct-labeled with PlatinumBright ™405.
Kreatech™ FISH probes do not contain Cot-1 DNA. Hybridization efficiency is therefore increased and background, due to unspecific binding, is highly reduced.