IVD Cell media

ivd-cell-media
KREAvital Prenatal Medium: Medium for Human Amniotic Fluid and Chorionic Villi Samples
The in vitro cultivation of amniotic fluid cells and chorionic villi is an essential part of every diagnostic cytogenetics laboratory, since the preparation of metaphase chromosome spreads is dependent upon obtaining cells in division. Amniocentesis and chorionic villi sampling are the major invasive diagnostic procedures used for the detection of fetal chromosomal abnormalities. KREAvital Prenatal Medium is specifically optimized for the primary culture of human amniotic fluid cells and chorionic villi samples used in prenatal diagnostic testing.
KBI-90010 and KBI-90011: The supplement is supplied frozen and contains serum.
KBI-90012 and KBI-92012: The medium is supplied frozen and contains serum, glutamine and antibiotics.
KBI-90013: The medium is a specialized version of KREAvital Prenatal Medium (Complete), as it has enhanced buffering capacity both in open (CO2) and closed systems; it has increased metaphase yeld of a highly pure amniotic cell population, yielding less epithelial cells; it has extended stability at 2 - 8°C. The medium is supplied frozen and contains serum, glutamine and antibiotics.

KREAvital Lymphocyte Karyotyping Medium (with/without PHA)
KBI-90020 and KBI-92020: KREAvital Lymphocyte Karyotyping Medium (KBI-90020 and KBI-92020) is intended for use in short-term cultivation of peripheral blood lymphocytes for chromosome evaluation. It is based on RPMI-1640 basal medium supplemented with L-Glutamine, fetal bovine serum and antibiotics (penicillin and streptomycin). KREAvital Lymphocyte Karyotyping Medium is supplied as frozen medium, which is ready for use after thawing and phytohaemagglutinin supplementation.
KBI-900021: KREAvital Lymphocyte Karyotyping Medium is intended for use in short-term cultivation of peripheral blood lymphocytes for chromosome evaluation. It is based on RPMI-1640 basal medium supplemented with L-Glutamine, fetal bovine serum, antibiotics (gentamicin) and Phytohaemagglutinin (PHA-M).

KREAvital Bone Marrow Karyotyping Medium
KBI-90030: KREAvital Bone Marrow Karyotyping Medium is intended for use in short-term cultivation of primary bone marrow cells for chromosome evaluation. It is based on RPMI-1640 basal medium supplemented with L-Glutamine, fetal bovine serum, and antibiotics (penicillin and streptomycin). The medium does not contain any mitogens or conditioned medium. KREAvital Bone Marrow Karyotyping Medium is supplied as frozen medium, which is ready for use after thawing.

KREAvital Myeloid Cell Medium
KBI-900031: KREAvital Myeloid Cell Medium is designed for culturing both lymphocytes and bone marrow cells originating from myeloid cells. It is a conditioned medium providing a higher mitotic index and superior chromosome morphology.
Cytogenetic analysis of human hematopoietic cells using bone marrow aspirates is a standard practice in hematology. Fresh cells or cells grown in short-term cultures often yield an insufficient number of mitotic cells and repeated aspirations are required. KREAvital Myeloid Cell Medium was developed to stimulate the proliferation of human hematopoietic cells from bone marrow as well as peripheral blood. KREAvital Myeloid Cell Medium is based on MEM-Alpha basal medium supplemented with L-Glutamine, fetal bovine serum, antibiotics (penicillin and streptomycin) and conditioned medium.
KREAvital Myeloid Cell Medium is supplied as frozen medium, which is ready for use after thawing.

Colchicine Solution (10 μg / ml, in PBS)
KBI-90050: Colchicine inhibits microtubule polymerization by binding to tubulin, one of the main constituents of microtubules. It arrests cells cultured in in vitro conditions at the mitotic phase, so that chromosomal spreads can be made for cytogenetic analyses.

Colcemid Solution (10 μg / ml, in PBS)
KBI-90051: Colcemid Solution is recommended for preparing good metaphase spreads in cytogenetic specimens of primary cultures established from tissue samples or cell lines. Treatment with Colcemid Solution causes disassembly of the microtubules and disruption of the cytoskeleton, and allows enrichment for cells in mitosis.
Colcemid is recommended for use in chromosome analysis during lymphocyte karyotyping and amniotic fluid cell chromosome analysis.

Potassium Chloride (0.075M)
KBI-90052: Potassium Chloride 0.075 M is a hypotonic solution, utilized for the preparation of blood lymphocyte chromosomes.

Sodium Citrate Solution (0.8%)
KBI-90054: Sodium Citrate Solution (0.8%) is a hypotonic solution, utilized for the preparation of blood lymphocyte chromosomes.

Phytohaemagglutinin liquid - For the stimulation of peripheral blood lymphocytes
KBI-90056: PHA-M is the mucoprotein form and is a crude extract used for the stimulation of cell proliferation in lymphocyte culture. PHA-M also has a powerful erythroagglutinating property and it was originally used for separating leukocytes from whole blood. (IVD)

Trypsin EDTA 10X (EDTA 0.2%, Trypsin 0.5%, in saline solution)
KBI-92055: Trypsin, an animal-derived product, is the most commonly used enzyme for harvesting cells in culture. Trypsin is a pancreatic serine protease (proteolytic) enzyme with specificity for peptide bonds involving the carboxyl group of the basic amino acids, Arginine and Lysine. Trypsin often contains a crude mixture of lipases, nucleases, polysaccharides and proteases extracted from Porcine pancreas.

Products

Products

KBI-90051
Colcemid Solution 10µg/ml in PBS
Country availability

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Media
In Vitro Diagnostic Use
100ml
Cell Medium
Ready-to-Use
Cell Medium
KBI-90050
Colchicine Solution 10µg/ml in PBS
Country availability

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Media
In Vitro Diagnostic Use
25 mL
Cell Medium
Ready-to-Use
Cell Medium
KBI-90030
KREAvital Bone Marrow Karyot.Medium100ml
Country availability

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Media
In Vitro Diagnostic Use
100ml
Cell Medium
Ready-to-Use
Cell Medium
KBI-90031
KREAvital Myeloid Cell Medium 100ml
Country availability

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Media
In Vitro Diagnostic Use
100ml
Cell Medium
Ready-to-Use
Cell Medium
KBI-90010
KREAvital Prenatal Medium (Basal) 90ml
Country availability

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Media
In Vitro Diagnostic Use
100ml
Cell Medium
Ready-to-Use
Cell Medium
KBI-90012
KREAvital Prenatal Medium (Compl)100ml
Country availability

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Media
In Vitro Diagnostic Use
100ml
Cell Medium
Ready-to-Use
Cell Medium
KBI-90011
KREAvital Prenatal Medium (Suppl)10ml
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Media
In Vitro Diagnostic Use
100ml
Cell Medium
Ready-to-Use
Cell Medium
KBI-90013
KREAvital PrenatalMediumPLUS(Compl)100ml
Country availability

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Media
In Vitro Diagnostic Use
100ml
Cell Medium
Ready-to-Use
Cell Medium
KBI-90021
Lymph.Karyot.Medium (incl.PHA) 100ml
Country availability

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Media
In Vitro Diagnostic Use
100ml
Cell Medium
Ready-to-Use
Cell Medium
KBI-90020
Lymph.Karyot.Medium (w/o PHA)100ml
Country availability

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Media
In Vitro Diagnostic Use
100ml
Cell Medium
Ready-to-Use
Cell Medium
KBI-90052
Potassium Cloride (0.075M)
Country availability

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Media
In Vitro Diagnostic Use
100ml
Cell Medium
Ready-to-Use
Cell Medium
KBI-90054
Sodium Citrate Solution (0.8%)
Country availability

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Media
In Vitro Diagnostic Use
250ml
Cell Medium
Ready-to-Use
Cell Medium
KBI-92012
KREAvital Prenatal Medium (Complete)
Country availability

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Media
In Vitro Diagnostic Use
250ml
Cell Medium
Fluorescent
Ready-to-Use
Cell Medium
KBI-92020
Lymph.Karyot.Medium (w/o PHA) 500ml
Country availability

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Media
In Vitro Diagnostic Use
250ml
Cell Medium
Ready-to-Use
Cell Medium
KBI-92055
Trypsin EDTA 10x 100ml
Country availability

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Media
In Vitro Diagnostic Use
100ml
Cell Medium
Ready-to-Use
Cell Medium

Downloads

Downloads

KBI-90051

KBI-90050

KBI-90030

KBI-90031

KBI-90010

KBI-90012

KBI-90011

KBI-90013

KBI-90021

KBI-90020

KBI-90052

KBI-90054

KBI-92012

KBI-92020

KBI-92055

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