Western Blot: analysis of human skeletal muscle showing detection of calpain 3 proteins. Lane A, calpain 3 bands at 94 and 30 kD detected with CALP-2C4. Lane B, Calpain 3 bands at 94 and approximately 60 kD detected with CALP-12A2. Calpain: clone Calc3d/2C4 Calpain: clone Calp3c/12A2

Calpain Antibodies

calpain-antibodies

Antigen Background

The gene responsible for LGMD2A has been identified as the chromosome 15q15-encoded muscle-specific calcium-activated neutral protease, calpain 3. Calpain 3 enzyme is only stable in human muscle when homogenized in treatment buffer immediately after harvest (Anderson LVB et al. Am. J. of Pathol. 153(4), 1169-1179 (1998)), and in homogenates containing SDS and is therefore well suited for analysis by Western blot. NCL-CALP-2C4 reacts with the full-size calpain 3 (94kD) and an additional fragment (30kD) in human skeletal muscle. NCL-CALP-12A2 reacts with full-size protein plus apparent degradation products at approximately 60kD.

Products

Products

CALP-12A2
2.5ml NCL-CALP-12A2
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CALP-2C4
2.5ml NCL-CALP-2C4
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CALP-12A2

CALP-2C4

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