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Antigen Retrieval Buffers are intended for Heat Induced Epitope Retrieval (HIER) on formalin-fixed, paraffin-embedded tissue sections as part of an immunohistochemical procedure.
HIER using an appropriate pH solution improves the staining of some antibodies by exposing epitopes within tissue that has been masked during fixation. A universal HIER technique suitable for all epitopes does not exist.
A combination of different heating methods and epitope retrieval solutions may be used to optimize unmasking of antigens where this technique is recommended. HIER is not recommended for all antibodies.
Optimum conditions for epitope retrieval should be validated by the user, as these are dependant upon tissue, fixation and/or primary antibody. The 10x solution is supplied in 1000 mL volume, sufficient to prepare 10 L of working solution.