Invasive breast carcinoma: immunohistochemical staining for Cytokeratin 7. Note the intense staining of invasive tumor cells. Cytokeratin 7: clone RN7

Cytokeratin 7

cytokeratin-7

Antigen Background

Cytokeratins are intermediate filament proteins present in epithelial cells. They are expressed in a tissue-specific manner in normal organs and the tumors that arise from them. Cytokeratin 7 belongs to the neutral basic type B subfamily of cytokeratins. Its distribution is confined to glandular and transitional epithelia. Cytokeratin 7 is reported to be expressed in abundance in cultured bronchial and mesothelial cells but only at lower levels in cultured epidermal cells. The predicted amino acid sequence of this keratin has revealed a striking difference between this keratin and the type II keratins expressed in epidermal cells. Cytokeratin 7 has been reported in adenocarcinomas of the lung, breast, endometrium, ovary, thyroid as well as in carcinomas of the bladder and chromophobe renal cell carcinoma. Cytokeratin 7 and Cytokeratin 20 expression have been reported to show characteristic patterns on primary and metastatic lung and colorectal adenocarcinomas.

Product Specific Information

Where clone OV-TL 12/30 can produce unwanted staining of endothelial cells, clone RN7 does not stain these cell types. The choice of epitope retrieval, heat or enzyme, to provide the best result with clone OV-TL 12/30 should be determined by the user. Clones RN7 and OV-TL 12/30 react with the human cytokeratin intermediate filament protein (54 kD) identified as cytokeratin 7.

Disclaimer

Cytokeratin 7 is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains.

  • This item replaces CK7-OVTL-CECK7-OVTL-R-7CK7-560-L-CE-SCK7-560-L-SCK7-OVTL-R-7-CE
    CK7-560-L-CE
    1ml NCL-L-CK7-560
    RN7
    Liquid Concentrate
    P (HIER)
  • CK7-560-L
    1ml NCL-L-CK7-560
    RN7
    Liquid Concentrate
    P (HIER)
  • CK7-OVTL-L
    1ml NCL-L-CK7-OVTL
    OV-TL12/30
    Liquid Concentrate
    P (ENZYME/HIER)
  • CK7-OVTL-L-CE
    1ml NCL-L-CK7-OVTL
    OV-TL12/30
    Liquid Concentrate
    P (ENZYME/HIER)
  • PA0138
    30ml Cytokeratin 7 Bond RTU Primary
    RN7
    BOND RTU
    P (HIER)
  • PA0942
    7ml Cytokeratin 7 Bond RTU Primary
    RN7
    BOND RTU
    P (HIER)

Product Specifications

Product Specifications

CK7-560-L-CE
Tumor Differentiation
RN7
Liquid Concentrate
Yes
P (HIER)
Mono
Mouse
In Vitro Diagnostic Use
1ml
CK7-560-L
Tumor Differentiation
RN7
Liquid Concentrate
Yes
P (HIER)
Mono
Mouse
Research Use Only
1ml
CK7-OVTL-L
Tumor Differentiation
OV-TL12/30
Liquid Concentrate
No
P (ENZYME/HIER)
Mono
Mouse
Research Use Only
1ml
CK7-OVTL-L-CE
Tumor Differentiation
OV-TL12/30
Liquid Concentrate
No
P (ENZYME/HIER)
Mono
Mouse
In Vitro Diagnostic Use
1ml
PA0138
Tumor Differentiation
RN7
BOND RTU
Yes
P (HIER)
Mono
Mouse
In Vitro Diagnostic Use
30ml
PA0942
Tumor Differentiation
RN7
BOND RTU
Yes
P (HIER)
Mono
Mouse
In Vitro Diagnostic Use
7ml

Documents

Documents

CK7-560-L-CE
CK7-560-L
CK7-OVTL-L
CK7-OVTL-L-CE
PA0138
PA0942

Resources

Resources

Antigen Background

Cytokeratins are intermediate filament proteins present in epithelial cells. They are expressed in a tissue-specific manner in normal organs and the tumors that arise from them. Cytokeratin 7 belongs to the neutral basic type B subfamily of cytokeratins. Its distribution is confined to glandular and transitional epithelia. Cytokeratin 7 is reported to be expressed in abundance in cultured bronchial and mesothelial cells but only at lower levels in cultured epidermal cells. The predicted amino acid sequence of this keratin has revealed a striking difference between this keratin and the type II keratins expressed in epidermal cells. Cytokeratin 7 has been reported in adenocarcinomas of the lung, breast, endometrium, ovary, thyroid as well as in carcinomas of the bladder and chromophobe renal cell carcinoma. Cytokeratin 7 and Cytokeratin 20 expression have been reported to show characteristic patterns on primary and metastatic lung and colorectal adenocarcinomas.

Product Specific Information

Where clone OV-TL 12/30 can produce unwanted staining of endothelial cells, clone RN7 does not stain these cell types. The choice of epitope retrieval, heat or enzyme, to provide the best result with clone OV-TL 12/30 should be determined by the user. Clones RN7 and OV-TL 12/30 react with the human cytokeratin intermediate filament protein (54 kD) identified as cytokeratin 7.

Disclaimer

Cytokeratin 7 is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains.

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