Rectal adenocarcinoma biopsy stained manually with HI-3A UltraPlex panel: CD4 (purple), CD8 (green), FoxP3 (yellow), PD-1 (red). 20X magnification

HI-3A Multiplex Panel - Human CD4, CD8, FoxP3, PD-1

DESCRIPTION: Kit for fluorescent multiplex IHC staining of human CD4, CD8, FoxP3, PD-1, detection with 490, 550, 650, 750 nm fluors

SIZE: 5 slides

PRODUCT OVERVIEW: All components required to stain 5 multiplex slides plus a single negative control slide with simple two-step protocol.

1° Antibody Clone 1° Tag 2° Antibody 2° Fluor
CD4 EPR6855 UT019 Anti-UT019 CL750
CD8 EPR10640(2) UT015 Anti-UT015 CL490
FoxP3 1054C UT014 Anti-UT014 CL550
PD-1 EPR4877 UT016 Anti-UT016 CL650

Optimized for use on FFPE tissue sections.
Suitable for conventional imaging using defined filter sets
Additional negative control (secondary reagents alone) are available, see NEG01A.

APPLICATION: IHC-P. Multiplex fluorescent tissue staining of human FFPE tissue sections


  • Concentrated individual 1° antibody-Tags (0.5mg/ml) in PBS/15mM azide
  • Concentrated individual 2° antibody-fluor (0.5mg/ml) in PBS/15mM azide
  • Antibody diluent solution - PBS/1% BSA/15mM azide/0.2% Tween 20
  • Protein block Solution – 3% rabbit serum in PBS/15mM azide/0.1% Triton X


SHELF-LIFE: 6 months

For Research Use Only. Not For Use In Diagnostic Procedures.



HI-3A mxIF - CD4/CD8/FoxP3/PD-1
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