Purple Hematoxylin

DESCRIPTION: Purple hematoxylin counterstain for IHC staining

SPECIFICATION: This counterstain is intended to be used by laboratory professionals to stain routinely prepared paraffin-embedded tissue specimens (in vitro) to identify nuclei and cytoplasm.

SIZE: 50ml

CONCENTRATION: Ready to use, liquid

APPLICATIONS: IHC, ISH

STORAGE: Room temperature

SHELF-LIFE: See expiration date on label

PRECAUTIONS: For use by laboratory professionals. See MSDS for complete warnings, precautions, hazard and precautionary statements, and disposal information.

DIRECTIONS:
For use with appropriately fixed, paraffin-embedded, 5 micron tissue sections. Hematoxylin can be used as a counterstain in immunohistochemistry or in situ hybridization. Purple hematoxylin is recommended for use with blue chromogens. Blue hematoxylin is recommended for use with all other chromogens. 1. After immunostaining rinse slides thoroughly with deionized water. 2. Immerse slides in hematoxylin for 3 minutes. Avoid overstaining as this may obscure weak immunostaining 3. Rinse slides in deionized water for 1-3 minutes 4. Note: Bluing is not required and may cause fading of certain chromogens 5. Mount slides according to recommended mounting procedures for specific chromogens.

DISCLAIMER
For Research Use Only. Not For Use In Diagnostic Procedures.