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L'acide-alcool est utilisé en histologie et en cytologie pour enlever les colorants en excès et définir les noyaux cellulaires après coloration à l'hématoxyline par les méthodes H&E et PAP.
Afin de produire une bonne coloration H&E aux détails nucléaires précis, il est essentiel de différencier correctement certaines hématoxylines, faisant de l'acide-alcool une solution hautement efficace.
Disponible avec 1 % ou 0,5 % d'acide chlorhydrique dans un réactif composé d'alcool à 70 %, cet acide-alcool peut considérablement accroître la reproductibilité des résultats de coloration et permet la standardisation.
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